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Histopathology Recipe Formulary J to P Return to Main Formulary Index |
| JENNER STAIN - (Gurr) | |
|---|---|
| Jenner stain | 0.5 g |
| Pure methanol | 100 ml |
| Dissolve by stirring. | |
| . |
| KAISERLING'S FIXATIVE - (Gurr) | |
|---|---|
| Potassium acetate | 100 g |
| Glycerol | 200 ml |
| Water | 1000 ml |
| KOPELOFF AND BEERMAN STAIN (see Gram) - (Gurr) | |
|---|---|
| . |
| LACTOPHENOL - (Gurr) | |
|---|---|
| Lactic acid | 25 ml |
| Glycerol | 50 ml |
| Distilled water | 25 ml |
| Phenol, cryst | 25 gm |
| Warm on a water bath until the phenol has gone into solution: then store in a dark glass bottle. |
| LACTO-FUCHSIN - (Gurr) | |
|---|---|
| Acid fuchsin | 0.1 g |
| Lactic acid | 100 ml |
| . |
| LEISHMAN STAIN - (Gurr) | |
|---|---|
| Leishman stain | 0.15 g |
| Pure methanol | 100 ml |
| May be dissolved by any of three methods: (a) place dye and alcohol in a flask, plug neck with cotton wool and warm in a water bath for 15 minutes with occasional shaking; (b) triturate dye and methanol in a mortar for 15 minutes; (c) stirring cold for 2 hours. The solution improves as a granule stain with keeping. |
| LIGHT GREEN - (Masson) | |
|---|---|
| Light green | 2 g |
| Glacial acetic acid | 2 ml |
| Water | 100 ml |
| . |
| LIGHT GREEN IN CLOVE OIL (or Cellosolve) - (Gurr) | |
|---|---|
| Light green | 1 g |
| Clove oil or cellosolve | 100 ml |
| Heat on water bath or electric oven or hotplate; cool then filter. |
| LISSAMINE FAST RED B (Picro-Lissamine) - (Lendrum) | |
|---|---|
| Lissamine fast red B 1% aqueous | 75 ml |
| Picric acid 1% aqueous | 25 ml |
| . |
| LOEFFLER'S FLAGELLA MORDANT - (Gurr) | |
|---|---|
| Tannic acid 20% aqueous solution | 100 ml |
| Ferrous sulphate crystals | 20 g |
| Basic fuchsin 10% in alcohol | 10 ml |
| Water | 40 ml |
| Dissolve iron salt in water by warming and add remainder; do not filter. |
| LOEFFLER'S FLAGELLA STAIN - (Gurr) | |
|---|---|
| Basic fuchsin 10% in alcohol | 20 ml |
| Aniline water 3% | 80 ml |
| . |
| LOEFFLER'S METHYLENE BLUE - (Gurr) | |
|---|---|
| Methylene blue | 0.5 g |
| 1% potassium hydroxide solution | 1 ml |
| Ethanol, absolute | 30 ml |
| Water | 100 ml |
| Warm the water to about 50 degrees C., stir in methylene blue and add other ingredients; filter. |
| LUGOL'S IODINE - (Gurr) | |
|---|---|
| Iodine | 1 g |
| Potassium iodide | 2 g |
| Water | 100 ml |
| Add iodine and iodide to about 25 ml water and when solution is complete add remainder of water. |
| LUXOL FAST BLUE-CRESYL FAST VIOLET - (Kluver & Barrera) | |
|---|---|
| Solution A | |
| Luxol Fast Blue | 1 g |
| 95% ethanol | 1 litre |
| Acetic acid 10% | 5 ml |
| Mix and filter. | |
| Solution B | |
| Cresyl Fast Violet | 1 g |
| Water | 1 litre |
| Before using add 5 drops 10% acetic acid to every 30 mls and filter. |
| MacCONKEY'S CAPSULE STAIN - (Gurr) | |
|---|---|
| Dahlia | 0.5 g |
| Methyl green | 1.5 g |
| Water | 100 ml |
| Dissolve and add: | |
| Basic fuchsin 10% alcohol solution | 10 ml |
| Water | 90 ml |
| Filter; stand 2 weeks before use, keeps about 6 months |
Use the Microscope button to return to the stain Index at the top of the page.
| McFADYEAN'S METHYLENE BLUE STAIN - (Gurr) | |
|---|---|
| This is a 'ripened' Loeffler's methylene blue. Loeffler's methylene blue is exposed to light and air for several months until it acquires a purplish tinge. |
| MALLORY'S ANILINE BLUE-ORANGE (see Aniline Blue-Orange) - (Gurr) | |
|---|---|
| . |
| MALLORY'S PHOSPHOTUNGSTIC HAEMATOXYLIN - (Gurr) | |
|---|---|
| Haematoxylin | 1 g |
| Water | 800 ml |
| 10% Phosphotungstic acid aq. soln. | 200 ml |
| Dissolve haematoxylin in a little of the water by heating and mix. Ripen by standing several weeks, or artificially by adding 12.5 ml. of a 1% solution of potassium permanganate. |
| MALLORY-HEIDENHAIN'S (Rapid One Step Stain) - (Gurr) | |
|---|---|
| Phosphotungstic acid | 1 g |
| Orange G. | 2 g |
| Aniline blue | 1 g |
| Acid fuchsin | 3 g |
| Distilled water | 200 ml |
| Add ingredients individually to the water and stir to dissolve after each addition. |
| MANN'S STAIN - (Gurr) | |
|---|---|
| Methyl blue 1% aqueous solution | 35 ml |
| Eosin 1% | 45 ml |
| Water | 100 ml |
| MANN'S DIFFERENTIATING ALCOHOL - (Gurr) | |
|---|---|
| Ethanol, 70% | 100 ml |
| Orange G. 7% aqueous | 4 ml |
| MASSON STAIN (see Ponceau Fuchsin) - (Gurr) | |
|---|---|
| MAY-GRUNWALD STAIN - (Gurr) | |
|---|---|
| May-Grunwald stain | 0.25 g |
| Pure methanol | 100 ml |
| Dissolve by trituration; shaking or stirring. |
| METHYL GREEN-ORANGE G. (Kardos Stain) - (Gurr) | |
|---|---|
| Kardos mixture | 0.5 g |
| Pure methanol | 100 ml |
| Dissolve on an electric hot plate or by stirring. |
| METHYL GREEN-PYRONINE - (Jordan & Baker) | |
|---|---|
| (i) Prepare a 0.5% aqueous methyl green solution and repeatedly extract with chloroform until chloroform extract is nearly colourless; at least 8 extractions are needed. | |
| (ii) Buffer Solution pH 4.8
Acetic Acid N/5 Sodium acetate M/t |
81 ml
119 ml |
| (iii) Stain
Pyronine Y 0.5% aqueous |
37 ml |
| Methyl green 0.5% (extracted as above) | 13 ml |
| Buffer solution | 50 ml |
| Solution (iii) keeps well for four months. |
| METHYL GREEN-PYRONINE - (Pappenheim) | |
|---|---|
| Methyl green 2% aqueous | 4 ml |
| Pyronine Y 5% aqueous | 7 ml |
| Distilled water | 100 ml |
| METHYL VIOLET 6B - (Jensen) | |
|---|---|
| Methyl violet 6B | 5 g |
| Water | 1,000 ml |
| Dissolve and filter. |
| METHYL VIOLET - (Kopelhoff & Beerman) | |
|---|---|
| Methyl violet 6B (or crystal violet) 1% aqueous | 30 ml |
| Sodium bicarbonate 5% solution | 8 ml |
| Should be freshly prepared every 3 or 4 days. |
| METHYLENE BLUE-FUCHSIN - (Gray) | |
|---|---|
| A. 1% aqueous methylene blue | 50 ml |
| Methanol, absolute | 50 ml |
| B. 1% aqueous basic fuchsin | 25 ml |
| Methanol, absolute | 25 ml |
| Dissolve dyes and mix A with B. |
| MUCICARMINE - (Gurr) | |
|---|---|
| Mucicarmine | 2.5 g |
| Ethanol 50% | 100 ml |
| Raise to boiling point in water bath, allow to boil 3 minutes, cool and filter. |
| MULLER'S FIXATIVE - (Gurr) | |
|---|---|
| Potass. dichromate | 25 g |
| Sodium sulphate | 10 g |
| Water | 1,000 ml |
| MUIR'S CAPSULE STAIN MORDANT - (Gurr) | |
|---|---|
| Saturated mercuric chloride solution (about 6%) | 20 ml |
| Tannic acid 20% solution | 20 ml |
| Saturated potash. alum solution (about 10%) | 50 ml |
| NEISSER METHYLENE BLUE - (Gurr) | |
|---|---|
| Methylene blue 2% aqueous solution | 10 ml |
| Ethanol, absolute | 4 ml |
| Glac. acetic acid | 10 ml |
| Water | 180 ml |
| NEISSER BISMARCK BROWN - (Gurr) | |
|---|---|
| Bismarck brown | 1 g |
| Distilled water | 500 ml |
| Heat water to about 60 degrees C., add dye and dissolve. |
| NEISSER CRYSTAL VIOLET - (Gurr) | |
|---|---|
| Crystal violet | 1 g |
| Ethanol, absolute | 10 ml |
| Water | 300 ml |
| NEISSER CHRYSOIDIN - (Gurr) | |
|---|---|
| Chrysoidin | 1 g |
| Water | 300 ml |
| NEUROGLIA MORDANT - (Anderson's) | |
|---|---|
| 1. Sodium sulphite | 5 g |
| Oxalic acid | 2.5 g |
| Potassium iodide | 5 g |
| Iodine | 2.5 g |
| Distilled water | 100 ml |
| Dissolve and add 5 ml glacial acetic acid | |
| 2. Ferric chloride (hydrated) 30% aqueous | 5 ml |
| Water | 95 ml |
| For use mix equal volumes of Nos. 1 and 2. |
| NEUTRAL RED - (Jensen) | |
|---|---|
| Neutral red | 1 g |
| Acetic acid 1% | 2 ml |
| Water | 1,000 ml |
| Dissolve and filter |
| NEUTRAL RED-LIGHT GREEN - (Twort) | |
|---|---|
| Neutral red-light green | 1 g |
| Clean sand | 10 g |
| iso-Propyl alcohol 60% | 100 ml |
| Grind all in a mortar for 5 minutes; filter. |
| NEWMAN STAIN (Modified) - (Gurr) | |
|---|---|
| Methylene blue | 1 g |
| 95% ethanol | 54 ml |
| Tetrachlorethane | 40 ml |
| Glacial acetic acid | 6 ml |
| Mix alcohol and tetrachlorethane, warm in water bath to 55 degrees C. Add methylene blue and shake to dissolve; cool and add acetic acid | |
| Add basic fuchsin 1% in absolute ethanol | 8 ml |
| Filter and keep well stoppered. |
| NIGROSINE - (Gurr) | |
|---|---|
| Nigrosine 5% aqueous solution. |
| NILE BLUE SULPHATE - (Gurr) | |
|---|---|
| Nile blue | 1 g |
| Sulphuric acid pure | 0.5 ml |
| Water | 100 ml |
| Boil gently for 2 hours, occasionally making up volume with water; cool, filter and make up to 100 mls, filter before use. |
| NUCLEAR FAST RED - (Kernechtrot) | |
|---|---|
| Nuclear fast red (Michrome No. 1022) | 0.1 g |
| Aluminium sulphate | 5 g |
| Water | 100 ml |
| Boil to dissolve. |
| OIL RED 4B STOCK SOLUTION - (Gurr) | |
|---|---|
| Prepare saturated solution as in the case of Oil Red O. |
| OIL RED O STOCK SOLUTION - (Gurr) | |
|---|---|
| Oil Red O | 0.5 g |
| iso-Propyl alcohol | 100 ml |
| Form saturated solution by warming in water bath or electric hot plate then filter |
| ORANGE FUCHSIN - (Gurr) | |
|---|---|
| Acid fuchsin 10% aqueous | 10 ml |
| Orange G, 3% aqueous | 200 ml |
| Distilled water | 30 ml |
| Absolute alcohol | 60 ml |
| ORCEIN - (Modified Unna) | |
|---|---|
| Orcein | 1 g |
| Hydrochloric acid, pure | 1 ml |
| Absolute ethanol | 100 ml |
| Shake to dissolve, or stand overnight; filter. |
| PAL'S SOLUTION - (Gurr) | |
|---|---|
| Oxalic acid | 1 g |
| Sodium sulphite | 1 g |
| Water | 200 ml |
| PANCHROME STAIN - (Pappenheim) | |
|---|---|
| Methylene blue | 1.0 g |
| Toluidine blue | 0.5 g |
| Azur 1 | 1.0 g |
| Methylene violet | 0.5 g |
| Eosin Y | 0.75 g |
| Pure methanol | 250 ml |
| Glycerol | 200 ml |
| Acetone | 50 ml |
| Place all except in a flask and warm in a water bath or an electric hot plate for 20 minutes; cool, add acetone, shake well and filter. |
| PAPANICOLAOU STAIN - (EA36) | |
|---|---|
| Eosin Y 10% aqueous solution | 11.25 ml |
| Light green SF 10% aqueous solution | 2.25 ml |
| Bismarck brown 1% alcoholic solution | 25 ml |
| Ethanol absolute | 450 ml |
| Phosphotongstic acid 10% aq. solution | 10 ml |
| Saturated aq. lithium carbonate | 0.3 ml |
| PAPANICOLAOU OG6 STAIN - (Gurr) | |
|---|---|
| Orange G satd. aqueous soln. | 32.5 ml |
| Phosphotungstic acid 10% aq. solution | 0.75 ml |
| Ethanol 95% | 475 ml |
| PASINI STAIN - (Gurr) | |
|---|---|
| Water blue-orcein | 30 ml |
| Eosin BA 2% in 50% alcohol | 30 ml |
| Acid fuchsin 20% aqueous | 4 ml |
| Glycerol | 25 ml |
| PHLOROGLUCINOL - (Gurr) | |
|---|---|
| Phloroglucinol | 1 g |
| Water | 100 ml |
| PHLOXINE - (Lendrum) | |
|---|---|
| Phloxine | 0.5 g |
| Calcium chloride | 0.5 g |
| Water | 100 ml |
| PIANESE STAIN - (Gurr) | |
|---|---|
| Malachite green | 0.5 g |
| Acid fuchsin | 0.1 g |
| Martius yellow | 0.01 g |
| Ethanol, absolute | 50 ml |
| Water | 150 ml |
| PICRIC ALCOHOL - (Masson) | |
|---|---|
| Picric acid saturated in 70% ethanol | 60 ml |
| Alcohol 95% | 30 ml |
| PICRO ANILINE BLUE - (Gurr) | |
|---|---|
| Aniline blue water soluble | 0.1 g |
| Satd. aqueous picric acid | 100 ml |
| PICRO-FORMOL-ALCOHOL - ("Rossman's Fluid") | |
|---|---|
| Picric acid satd. alcholic soln. | 90 ml |
| Formalin | 10 ml |
| PICRO FUCHSIN - (see Van Gieson) | |
|---|---|
| PICRO-PONCEAU S - (Gurr) | |
|---|---|
| Ponceau S 1% aq. solution | 10 ml |
| Saturated aqueous picric acid | 90 ml |
| Acetic acid 2% | 2 ml |
| POLYCHROME-METHYLENE BLUE - (Unna) | |
|---|---|
| Methylene blue | 1 g |
| Potass. carbonate | 1 g |
| Ethanol, absolute | 20 ml |
| Water | 100 ml |
| Dissolve methylene blue in water, add potass, carbonate and alcohol, place in water bath and evaporate to 100 ml |
| POLYCHROME TOLUIDINE BLUE - (Martinotti) | |
|---|---|
| Lithium carbonate | 0.5 g |
| Toluidine Blue | 1.0 g |
| Water | 75 ml |
| Glycerol | 20 ml |
| 95% ethanol | 5 ml |
| Warm water, stir in lithium, then toluidine
blue, dissolve and add glycerin-alcohol.
Ripen for one month. |
| PONCEAU FUCHSIN - (Masson) | |
|---|---|
| Acid fuchsin | 0.3 g |
| Ponceau de xylidine | 0.7 g |
| G;acoa; acetic acid | 1 ml |
| Water | 100 ml |
| PONDER'S STAIN - (Gurr) | |
|---|---|
| Toluidine blue | 0.1 g |
| Acetic acid glacial | 5 ml |
| Ethanol, absolute | 10 ml |
| Water | 485 ml |
| PUGH'S STAIN - (Gurr) | |
|---|---|
| Toluidine blue | 1 g |
| Acetic acid glacial | 25 ml |
| Ethanol, absolute | 10 ml |
| Water | 465 ml |
| RANDOLPH'S BLOOD DILUTING FLUID - (Gurr) | |
|---|---|
| A. Methylene blue | 0.1 g |
| Propylene glycol | 100 ml |
| Water | 100 ml |
| B. Phloxine | 0.1 g |
| Propylene glycol | 100 ml |
| Water | 100 ml |
| Mix equal volumes of A & B immediately before use; the mixture remains usable for 4 hours |
| REGAUD'S FIXATIVE - (Gurr) | |
|---|---|
| Potass. dichromate 3% solution | 100 ml |
| Formalin (neutralised by shaking with magnesium carbonate) | 25 ml |
| REGAUD'S HAEMATOXYLIN - (Gurr) | |
|---|---|
| Haematoxylin | 1 g |
| Ethanol, absolute | 10 ml |
| Glycerol | 10 ml |
| Water | 80 ml |
| Allow to ripen for 3 weeks, or, prepare with 10 ml. ripened 10% alcoholic haematoxylin instead of first two ingredients. |
| RONGALITE WHITE - (Leuco Methylene Blue) | |
|---|---|
| Methylene blue 0.5% aqueous | 100 ml |
| Hydrochloric acid pure | 5 drops |
| Rongalite | 3 g |
| Add acid to methylene blue solution, warm and add rongalite; cool and filter. Stable for a few days. |
| SAFFRON - (Gurr) | |
|---|---|
| Saffron | 2 g |
| Water | 100 ml |
| Place in a flask and boil for an hour in a water bath. Filter and add: | |
| Formalin | 1 ml |
| Tannic acid 5% | 1 ml |
| Note: Saffron, whose, solution deteriorates after a few weeks, should not be confused with the cationic dye, safranin commonly used in histology. |
| SAFRANIN ALCOHOLIC - (Gurr) | |
|---|---|
| Safranin | 1 g |
| Water | 50 ml |
| Dissolve and add: | |
| Ethanol, absolute | 50 ml |
| Filter. |
| SAFRANIN AQUEOUS - (Gurr) | |
|---|---|
| Safranin | 1 g |
| Water | 100 ml |