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Histopathology Method Manual - Routine Staining Procedures

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TABLE OF CONTENTS

AMYLOID 
1.1 ALKALINE CONGO RED FOR AMYLOID

CONNECTIVE TISSUES 
2.1 VERHOEFF-VAN GIESON STAIN FOR ELASTIC FIBRES 
2.2 MASSON'S TRICHROME STAIN FOR COLLAGEN 
2.3 GOMORI'S ONE-STEP TRICHROME STAIN 
2.4 VAN GIESON TECHNIQUE FOR COLLAGEN 
2.5 RETICULIN FIBRES 
2.6 GORDON & SWEETS' METHOD FOR RETICULIN FIBRES 
2.7 MALLORY'S PHOSPHOTUNGSTIC ACID HAEMATOXYLIN (PTAH) FOR MUSCLE STRIATIONS 
2.8 SIRIUS RED FOR COLLAGEN AND BILE PIGMENTS IN LIVER TISSUE.
 

FIBRIN 
3.1 MARTIUS YELLOW - BRILLIANT CRYSTAL SCARLET BLUE (MSB) TECHNIQUE - FOR FIBRIN
3.2 LENDRUM'S PICRO-MALLORY METHOD FOR FIBRIN
 

LIPIDS 
4.1 OIL RED O FOR THE DEMONSTRATION OF LIPIDS 
4.2 NILE BLUE SULPHATE METHOD FOR ACIDIC AND NEUTRAL LIPIDS 
 

MICRO-ORGANISMS 
5.1.1 CONTROL FOR GRAM STAIN 
5.1.2 GRAM STAIN FOR BACTERIA 
5.2 DIFF-QUIK STAIN FOR CAMPYLOBACTER-LIKE ORGANISMS. 
5.3 ZIEHL - NEELSEN STAIN FOR TUBERCLE BACILLI (A.F.B.) 
5.4 WADE - FITE FOR DEMONSTRATION OF MYCOBACTERIUM LEPRAE 
5.5 WARTHIN STARRY METHOD FOR SPIROCHAETES AND DONOVAN BODIES 
5.6 GIEMSA STAIN FOR DONOVAN BODIES AND PARASITES 
5.7 PHLOXINE-TARTRAZINE TECHNIQUE FOR VIRAL INCLUSIONS 
5.8 ORCEIN STAIN FOR HEPATITIS B ANTIGEN (HBSAG) IN LIVER TISSUE 
5.9 VICTORIA BLUE - NUCLEAR FAST RED 
5.10 GROCOTT - GOMORI METHENAMINE SILVER METHOD FOR FUNGI. 
5.11 GRAM WEIGERT STAIN FOR PNEUMOCYSTIS CARINII AND GRAM-POSITIVE ORGANISMS 
 

TISSUE CARBOHYDRATES - GLYCOGEN AND MUCINS 
6.1 PERIODIC ACID SCHIFF (PAS )FOR THE DEMONSTRATION OF GLYCOGEN 
6.2 PERIODIC ACID SCHIFF (PAS) WITH DIASTASE FOR THE ENZYME EXTRACTION OF GLYCOGEN
6.3 ALCIAN BLUE TECHNIQUE FOR ACID MUCINS 
6.4 COMBINED ALCIAN BLUE PAS TECHNIQUE FOR ACID AND NEUTRAL MUCINS 
6.5 COMBINED ALCIAN BLUE/DIASTASE/PAS FOR ACID & NEUTRAL MUCINS
6.6 BEST'S CARMINE TECHNIQUE FOR GLYCOGEN 
6.7 SOUTHGATE'S MUCICARMINE FOR EPITHELIAL MUCIN 
 

NERVOUS TISSUE 
7.1 BIELSCHOWSKY'S SILVER STAIN FOR AXONS (MODIFIED) 
7.2 LUXOL FAST BLUE TECHNIQUE FOR MYELIN 
7.3 PAS - ORANGE G METHOD FOR ANTERIOR PITUITARY CELLS. 
 

PIGMENTS AND MINERALS 
8 1 REMOVAL OF FORMALIN PIGMENT 
8 2 REMOVAL OF MERCURY PIGMENT 

    DEMONSTRATION OF BILE 
8 3.1 DEMONSTRATION OF BILE AND HAEMATOIDIN 
8.3.2 FOUCHET'S REACTION FOR BILE PIGMENTS 
    DEMONSTRATION OF FERRIC IRON 
8.4.1 PERL'S PRUSSIAN BLUE FOR FERRIC IRON 
8.5 REMOVAL OF MELANIN PIGMENTS (BLEACH) 
8.6 MASSON - FONTANA METHOD FOR MELANIN
8.7 SCHMORL'S REACTION FOR MELANIN 
8.8 MODIFIED VON KOSSA'S METHOD FOR CALCIUM 
8.9 TIMM'S COPPER STAIN - (MODIFIED) 
8.10 DEMONSTRATION OF URIC ACID AND URATES 
 

 INTRACELLULAR NEUROSECRETORY GRANULES 
9.1 ALKALINE DIAZO TECHNIQUE FOR ARGENTAFFIN CELL GRANULES. 
9.2 GRIMELIUS SILVER METHOD FOR ARGYROPHIL CELLS, ESPECIALLY A (A2 ) CELLS OF THE PANCREAS 
 

HAEMATOLOGICAL CELLS 
10.1 METHYL GREEN - PYRONIN TECHNIQUE FOR PLASMA CELLS 
10.2 HITCHCOCK-EHRICH TECHNIQUE FOR PLASMA CELLS 
10.3 UNNA'S METHOD FOR MAST CELLS 
10.4 TOLUIDINE BLUE METHOD FOR MAST CELLS AND OTHER METACHROMATIC SUBSTANCES 
10.5 LUNA'S METHOD FOR ERYTHROCYTES AND EOSINOPHILS. 
10.6 DUNN THOMPSON METHOD FOR HAEMOGLOBIN 
10.7 OKAJIMA'S METHOD FOR HAEMOGLOBIN 
 

 MISCELLANEOUS 
11.1 ATTWOOD'S METHOD FOR AMNIOTIC FLUID EMBOLISM 
11.2 MALLORY BODIES (ALCOHOLIC HYALINE)  


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Note: Most files presented on these pages are extracted from the Histopathology Manuals of the Royal Darwin Hospital, Pathology Department and are presented with permission of the management. Thanks and credit for the creation of these manuals goes to the staff of the department.
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Histopathology Text Books from Amazon.co.uk 

(a sample of the listing - click on any textbook link to go to Amazon.co.uk)
 
 

Histological Typing of Ovarian Tumours by R. Scully 


Recent Advances in Histopathology Vol 18 by David G. Lowe(Editor), James C.E. Underwood (Editor)  Usually dispatched in 2-3 days
Churchill Livingstone Paperback


Wheater's Basic Histopathology by H. George Burkitt, et al  Usually dispatched in 24 hours
Churchill Livingstone Paperback


An Atlas of Histopathology of Skin Diseases (Encyclopedia of Visual Medicine Series) by David T. Shum, et al 


Atlas of Surgical Pathology of the Male Reproductive Tract (Atlases in Diagnostic Surgical Pathology) by Jae Y. Ro, et al 


Breast Specimen Radiography : Needle Localization and Radiographic Pathologic Correlation by Eva Rubin, Jean F. Simpson 


Gastrointestinal Pathology by Parakrama Chandrasoma 


Gastrointestinal Pathology : An Atlas and Text by Cecilia M. Fenoglio-Preiser(Editor) 


Histology for Pathologists by Stephen S. Sternberg(Editor)    "-1"> Lippincott-Raven Publishers Hardcover


Inflammation : A Review of the Process by Henry O. Trowbridge, Robert C. Emling 


Lever's Histopathology of the Skin by Walter F. Histopathology of the Skin Lever(Editor), rosalie Elenitsas 


Mucosal Biopsy of the Gastrointestinal Tract (5th Ed (Major Problems in Pathology, Vol 3) by Richard Whitehead 


Pathology of the Gastrointestinal Tract by Si-Chun Ming(Editor), Harvey Goldman (Editor) 


Pathology of the Prostate (Major Problems in Pathology, Vol 34) by Christopher S. Foster, David G. Bostwick

Phospholipase A2 in Clinical Inflammation : Molecular Approaches to Pathophysiology (Pharmacology and Toxicology : Basic and Clinical Aspects) by Keith B. Glaser(Editor), et al 


Textbook of Dermatopathology by Raymond L. Barnhill(Editor), Raymond Barnhill 


Acne and Rosacea by G. Plewig, A.M. Kligman 


Alopecia Areata by Wendy Thompson MA, Jerry Shapiro MD  Usually dispatched in 24 hours
The Johns Hopkins University Press Hardcover


An Atlas and Textbook of Malignant Lymphomas by P. van Heerde MD PhD, et al 


ATLAS FORENSIC HISTOPATHOLOGY by Vanezis P 


Atlas of Biopsy Histopathology for Heart and Lung Transplantation by M. Billingham, et al 


Atlas of Correlative Surgical Neuropathology and Imaging by G.S. Rutherford, R.H. Hewlett 


An Atlas of Cytopathology of the Head and Neck by Genevieve Warner Learmonth BCh BAO 


An Atlas of Cytopathology of the Head and Neck by Genevieve Warner Learmonth BCh BAO 


Atlas of Endometrial Histopathology by G. Dallenbach-Hellweg, H. Poulsen 


Atlas of Glomerular Histopathology by P M H Schillings, J Herman Schuurmans Stekhoven 


Atlas of Histopathology of the Uterine Cervix


Basic Histopathology for Medical Laboratory Students by Antony S.-Y. Leong(Editor) 


Biopsy of Bone in Internal Medicine by R. Bartl, B. Frisch 


Biopsy Pathology of the Breast by J. Sloane 


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